Potassium bromate(VA Medical Center)(Potassium bromate(VA Medical Center)) - 药物靶点：Bcl-2 x caspase 3_在研适应症：乳腺癌_专利_临床

概要

基本信息

药物类型

小分子化药

别名-

靶点

Bcl-2 x caspase 3

作用机制

Bcl-2抑制剂(细胞凋亡调控因子Bcl-2抑制剂)、caspase 3抑制剂(半胱天冬酶-3抑制剂)

治疗领域

肿瘤皮肤和肌肉骨骼疾病

在研适应症

乳腺癌

非在研适应症-

原研机构

VA Medical Center

在研机构

VA Medical Center

非在研机构-

最高研发阶段临床前

首次获批日期-

最高研发阶段(中国)-

特殊审评-

关联

100 项与 Potassium bromate(VA Medical Center) 相关的临床结果

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100 项与 Potassium bromate(VA Medical Center) 相关的转化医学

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100 项与 Potassium bromate(VA Medical Center) 相关的专利（医药）

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1

项与 Potassium bromate(VA Medical Center) 相关的文献（医药）

2021-08-01·Archives of Toxicology2区 · 医学

Validation of the in vitro comet assay for DNA cross-links and altered bases detection

2区 · 医学

Article

作者: Sanz-Serrano, Julen ; Muruzabal, Damián ; Olsen, Ann-Karin ; Azqueta, Amaya ; Sauvaigo, Sylvie ; Treillard, Bertrand ; López de Cerain, Adela ; Vettorazzi, Ariane

AbstractMechanistic toxicology is gaining weight for human health risk assessment. Different mechanistic assays are available, such as the comet assay, which detects DNA damage at the level of individual cells. However, the conventional alkaline version only detects strand breaks and alkali-labile sites. We have validated two modifications of the in vitro assay to generate mechanistic information: (1) use of DNA-repair enzymes (i.e., formamidopyrimidine DNA glycosylase, endonuclease III, human 8-oxoguanine DNA glycosylase I and human alkyladenine DNA glycosylase) for detection of oxidized and alkylated bases as well as (2) a modification for detecting cross-links. Seven genotoxicants with different mechanisms of action (potassium bromate, methyl methanesulfonate, ethyl methanesulfonate, hydrogen peroxide, cisplatin, mitomycin C, and benzo[a]pyrene diol epoxide), as well as a non-genotoxic compound (dimethyl sulfoxide) and a cytotoxic compound (Triton X-100) were tested on TK-6 cells. We were able to detect with high sensitivity and clearly differentiate oxidizing, alkylating and cross-linking agents. These modifications of the comet assay significantly increase its sensitivity and its specificity towards DNA lesions, providing mechanistic information regarding the type of damage.

100 项与 Potassium bromate(VA Medical Center) 相关的药物交易

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